miR-132 mediates cell permeability and migration by targeting occludin in high-glucose -induced ARPE-19 cells

This study investigated the effects and mechanisms of miR-132 related to permeability mobility human retinal pigment epithelium ARPE-19 cells in high-glucose (HG) condition. were cultured normal HG condition identified by immunofluorescence staining. Cell viability was assessed MTT assay, cell FITC-dextran assay wound healing assay. Different miRNA mRNA expression levels determined quantitative real-time polymerase chain reaction (RT-qPCR). The tight junction-related proteins Western blot immunofluorescence. interaction between occludin confirmed a dual-luciferase reporter We revealed that HG-treated exhibited significantly increased expression, decreased tight-junction markers including E-cadherin, permeability. Occludin is direct target miR-132, which could regulate viability, under through JAK/STAT3 signaling pathway. These are first data suggest may contribute progression diabetic retinopathy (DR) targeting effect on occudin pathway represent novel effective DR-treatment strategy.